| 刘庆坚,王玉珑,韩俊源,刘艳新.纳米微晶纤维素/槲皮素复合物的制备及抗氧化和抗癌性能的研究[J].中国造纸学报,2020,35(1):7-14 |
二维码（扫一下试试看！） |
| 纳米微晶纤维素/槲皮素复合物的制备及抗氧化和抗癌性能的研究 |
| Preparation of Cellulose Nanocrystal/Quercetin Nanocomposite and Its Anti-oxidation and Anti-cancer Activities |
| 投稿时间：2019-08-18 |
| DOI：10.11981/j.issn.1000-6842.2020.01.07 |
| 中文关键词: 纳米微晶纤维素（CNC） 槲皮素 纳米复合物 溶出度 抗氧化活性 抗癌性能 |
| Key Words:cellulose nanocrystals (CNC) quercetin nanocomposite dissolution rate anti-oxidation anti-cancer |
| 基金项目: |
|
| 摘要点击次数: 3665 |
| 全文下载次数: 2376 |
| 中文摘要: |
| 以纳米微晶纤维素（CNC）为载体，采用反溶剂重结晶法制备了CNC/槲皮素纳米复合物；采用傅里叶变换红外光谱（FT-IR）、透射电子显微镜（TEM）、X射线衍射仪（XRD）等对CNC/槲皮素纳米复合物进行表征，并对纳米复合物中槲皮素的溶出性能、抗氧化活性（羟基自由基和1,1-二苯基-2-苦基肼（DPPH）自由基的清除率）和抗癌性能（HepG2肝癌细胞，MTT比色法）进行研究。结果表明，形成CNC/槲皮素纳米复合物后，槲皮素被充分纳米化和稳定分散，由结晶态转变为无定形态；槲皮素的溶出性能得到显著提升，溶解120 min时，槲皮素的溶出度达到92.6%（槲皮素原料溶解120 min时的溶出度仅为4.7%）；体外抗氧化活性和HepG2细胞毒性研究表明，形成CNC/槲皮素纳米复合物后，槲皮素的羟基自由基和DPPH自由基清除率得到显著提高，抗癌效果得到明显改善。 |
| Abstract: |
| Cellulose nanocrystal (CNC) was used as carriers and quercetin as model drug to prepare CNC/quercetin nanocomposites via anti-solvent recrystallization process. The CNC/quercetin nanocomposite was characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM) and X-ray diffractometry (XRD),the dissolution rate, anti-oxidation and anti-cancer activities of the resultant nanocomposite were investigated. The results showed that quercetin was fully nanolized and well dispersed in CNC/quercetin nanocomposite, and changed from crystalline state to amorphous state. The dissolution rate of quercetin was significantly enhanced due to the formation of the CNC/quercetin nanocomposite,increased to 92.6% at 120 min, compared with the dissolution rate of original quercetin of 4.7%. Furthermore, in vitro anti-oxidation and anti-cancer activitiy studied results showed that hydroxyl radical and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radicals scavenging efficiency, as well as anti-cancer effect against HepG2 cells of guercetin were remarkably enhanced, based on the formation of the CNC/quercetin nanocomposites. |
| 查看全文 查看/发表评论 下载PDF阅读器 HTML |
