| 齐云赓,马明帅,郭怀泽,李海明.UPLC-PDA法检测桉木预水解液中5-羟甲基糠醛[J].中国造纸学报,2020,35(3):40-45 |
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| UPLC-PDA法检测桉木预水解液中5-羟甲基糠醛 |
| Determination of 5-Hydroxymethylfurfural in Pre-hydrolysate of Eucalyptus by UPLC-PDA Method |
| 投稿时间：2019-05-31 |
| DOI：10.11981/j.issn.1000-6842.2020.03.40 |
| 中文关键词: 5-羟甲基糠醛 超高效液相色谱 二极管阵列 预水解 桉木 |
| Key Words:5-hydroxymethylfurfural UPLC photo-diode array pre-hydrolysis Eucalyptus |
| 基金项目:国家自然科学基金（31370584）；2018年辽宁省高等学校创新人才项目。 |
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| 中文摘要: |
| 采用超高效液相色谱-二极管阵列检测器（UPLC-PDA）法检测桉木预水解液中5-羟甲基糠醛（5-HMF）。该方法检测过程中，样品无需复杂的预处理，即可在流动相为乙腈/水（20∶80，V/V）、淋洗速度为0.2 mL/min的条件下对桉木预水解液中各组分进行先洗脱分离，然后采用PDA检测器于波长283.55 nm处定量检测桉木预水解液中的5-HMF；其检出限为3.883 μg/L，相关系数为0.9998，重现检测相对标准偏差为0.2%，加标回收率为105.2%；该法具有样品预处理简单、检测快速和准确的特点，为更好地控制预水解条件、提高预水解液利用价值提供了一定的理论支撑。 |
| Abstract: |
| A method was developed to determine 5-hydroxymethylfurfural （5-HMF） in the pre-hydrolysate of Eucalyptus by Ultra Performance Liquid Chromatography-Photo-diode Array Detector （UPLC-PDA）. Without complicated pretreatment， different components in the samples were eluted and then separated with the mobile phase of acetonitrile/water （20/80，V/V） at flow rate of 0.2 mL/min， and then quantitatively detected with PDA detector at 283.55 nm. Detection limit， the correlation coefficient， relative standard deviation （RSD） and 5-HMF standard recovery rate of the determination method were 3.883 μg/L， 0.9998， 0.2% and 105.2%， respectively. The method developed had characteristics of simple sample preparation， fast determination and high accuracy. Finally， this method was used for fast determination of 5-HMF in the Eucalyptus pre-hydrolysate， which was beneficial to optimization of pre-hydrolysis conditions and utilization of pre-hydrolysate. |
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