| 朱鹤馨,汪思维,胡慧捷,赵铭,刘念,胡静,蒋云,邓海波.α-淀粉酶及其复合酶体系对淀粉酶解性能研究[J].中国造纸学报,2024,(3):87-95 |
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| α-淀粉酶及其复合酶体系对淀粉酶解性能研究 |
| Study on the Starch Enzymolysis Performance of α-Amylase and Its Complex Enzyme System |
| 投稿时间：2024-04-03 修订日期：2024-04-17 |
| DOI：10.11981/j.issn.1000-6842.2024.03.87 |
| 中文关键词: 变性淀粉 α-淀粉酶 复合酶体系 酶解效率 |
| Key Words:modified starch α-amylase complex enzymes enzymatic hydrolysis efficiency |
| 基金项目:国家十四五重点研发计划（2022YFC2105504）。 |
| 作者 | 单位 | 邮编 | | 朱鹤馨 | 江南大学生态纺织教育部重点实验室,江苏无锡,214122 | 214122 | | 汪思维 | 江南大学生态纺织教育部重点实验室,江苏无锡,214122 | 214122 | | 胡慧捷 | 江南大学生态纺织教育部重点实验室,江苏无锡,214122 | 214122 | | 赵铭 | 江南大学生物工程学院,江苏无锡,214122 | 214122 | | 刘念 | 江南大学无锡医学院,江苏无锡,214122 | 214122 | | 胡静 | 江南大学无锡医学院,江苏无锡,214122 | 214122 | | 蒋云 | 江南大学生态纺织教育部重点实验室,江苏无锡,214122 | 214122 | | 邓海波* | 江南大学生态纺织教育部重点实验室,江苏无锡,214122 | 214122 |
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| 中文摘要: |
| 研究了单一α-淀粉酶体系及α-淀粉酶与β-淀粉酶、普鲁兰酶、糖化酶组成的二元、三元、四元复合酶体系，对3种造纸常用变性淀粉（氧化淀粉、酶解淀粉、阳离子淀粉）的酶解性能，探究不同α-淀粉酶用量对淀粉分解率和DE值的影响。结果表明，在单一α-淀粉酶体系中，淀粉分解率达到90%时，阳离子淀粉酶解所需α-淀粉酶用量约为其他2种淀粉的4倍；酶解淀粉加入柠檬酸抑制Zn2+后，其淀粉分解率最大可提高37.4%；二元复合酶体系中，α-淀粉酶/β-淀粉酶对阳离子淀粉的分解率比α-淀粉酶/糖化酶及α-淀粉酶/普鲁兰酶分别高5.3%和8.7%；三元复合酶体系中，α-淀粉酶/糖化酶/β-淀粉酶对阳离子淀粉的分解率比α-淀粉酶/糖化酶/普鲁兰酶高1.2%。在将α-淀粉酶/糖化酶/β-淀粉酶/普鲁兰酶的四元复合酶体系应用于阳离子淀粉、酶解淀粉、氧化淀粉的酶解反应时，达到淀粉分解率80%所需α-淀粉酶用量分别为单一α-淀粉酶体系的56%、38%和17%。 |
| Abstract: |
| This study investigated the enzymatic performance of single α-amylase and binary， ternary， and quaternary complex enzyme systems consisting of α-amylase with β-amylase， pullulanase and glucoamylase for three commonly used modified starches （oxidized starch， enzymatically hydrolyzed starch， and cationic starch）. The effect of different α-amylase dosages on starch enzymatic rate and DE value was explored. The results indicated that when the starch enzymatic rate reached 90% in single α-amylase system， the required amount of α-amylase for cationic starch enzymolysis was about 4 times than that of the other two kinds of starches. The enzymatic rate of enzymatically hydrolyzed starch could be increased by up to 37.4% when citric acid was added to inhibit zinc ions. In the binary complex enzyme system， the enzymatic rate of cationic starch by α-amylase/β-amylase was 5.3% and 8.7% higher than that by α-amylase/glucoamylase and α-amylase/pullulanase， respectively. In the ternary complex enzyme system， the enzymatic rate of cationic starch by α-amylase/glucoamylase/β-amylase was 1.2% higher than that by α-amylase/glucoamylase/pullulanase. In the application of α-amylase/glucoamylase/β-amylase/pullulanase tetrameric complex enzyme system to the enzymatic reactions of cationic starch， enzymatically hydrolyzed starch and oxidized starch， when the enzymatic rate of starch reached 80%， the required amount of α-amylase was 56%， 38% and 17% of that by single α-amylase system， respectively. |
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