| 刘雪勤,吕英仁,朱若楠,刘小红,王兴杰,任俊莉.超低浓酸预处理对麦草秸秆组分分离及纤维素酶水解效率的影响[J].中国造纸学报,2025,(2):1-9 |
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| 超低浓酸预处理对麦草秸秆组分分离及纤维素酶水解效率的影响 |
| Effects of Ultra-low Concentration Acid Pretreatment on the Separation of Wheat Straw Components and Cellulose Enzymolysis Efficiency |
| 投稿时间：2024-11-13 修订日期：2024-12-17 |
| DOI：10.11981/j.issn.1000-6842.2025.02.01 |
| 中文关键词: 麦草秸秆 甲酸 预处理 酶水解 |
| Key Words:wheat straw formic acid pretreatment enzyme hydrolysis |
| 基金项目:国家重点研发课题（2021YFC2101601）；国家自然科学基金国际合作项目（22361132543）。 |
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| 中文摘要: |
| 本研究以麦草秸秆为原料，探讨了超低浓酸种类及预处理条件对麦草秸秆组分分离及纤维素酶水解效果的影响。结果表明，甲酸预处理效果最佳，在甲酸浓度1 g/L、固液比1∶20（g∶mL）、预处理温度170 ℃、预处理时间30 min条件下，麦草秸秆纤维素保留率95.55%，半纤维素脱除率81.66%，木质素脱除率38.42%，水解液中木糖及低聚木糖总得率80.05%；预处理残渣经过72 h酶水解后，葡萄糖得率83.37%。通过物料平衡计算，该工艺下总糖（葡萄糖和木糖）得率大于95.32%。 |
| Abstract: |
| In this study， the effects of ultra-low concentration acid types and pretreatment conditions on the fractionation of wheat straw and enzymolysis were investigated. The results showed that formic acid pretreatment was the most effective. Under the pretreatment conditions of formic acid mass concentration of 1 g/L， the solid-liquid ratio of 1∶20， pretreatment temperature of 170 ℃， and time of 30 min， the 95.55% cellulose retention rate was achieved. The removal rate of hemicellulose and lignin were 81.66% and 38.42%， respectively， the total yield of xylose and xylo-oligosaccharide in the hydrolysate was 80.05%. In addition， the pretreatment residue obtained under this condition showed a glucose conversion of 83.37% after 72 h of enzymatic hydrolysis reaction. After two-step hydrolysis， the glucose and xylose yields was obtained to 95.32%. |
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