| 王一帆,孔令懿,李晓,于孟辉,王高升.复合蛋白质选择性吸附玉米秸秆水解液中木质素及机制研究[J].中国造纸学报,2026,41(2):95-105 |
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| 复合蛋白质选择性吸附玉米秸秆水解液中木质素及机制研究 |
| Study on the Selective Adsorption of Lignin from Corn Stover Hydrolysate Using Protein Complex and Its Mechanism |
| 投稿时间：2025-09-24 修订日期：2025-11-07 |
| DOI：10.11981/j.issn.1000-6842.2026.02.95 |
| 中文关键词: 玉米秸秆水解液 木质素去除 复合蛋白质 选择吸附 机制 |
| Key Words:corn stover hydrolysate lignin removal protein complex selective adsorption mechanism |
| 基金项目: |
| 作者 | 单位 | 邮编 | | 王一帆* | 天津科技大学轻工科学与工程学院,天津市制浆造纸重点实验室,天津,300457 | 300457 | | 孔令懿 | 天津科技大学轻工科学与工程学院,天津市制浆造纸重点实验室,天津,300457 | 300457 | | 李晓 | 天津科技大学轻工科学与工程学院,天津市制浆造纸重点实验室,天津,300457 | 300457 | | 于孟辉 | 天津科技大学轻工科学与工程学院,天津市制浆造纸重点实验室,天津,300457 | 300457 | | 王高升* | 天津科技大学轻工科学与工程学院,天津市制浆造纸重点实验室,天津,300457 | 300457 |
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| 中文摘要: |
| 本研究采用蛋清蛋白-大豆分离蛋白的复合物对玉米秸秆经水热预处理得到的水解液中木质素进行吸附处理，探讨了复合蛋白质选择性吸附水解液中木质素的效果与机制。结果表明，在反应温度95 ℃、pH值=5、复合蛋白质添加量1.5 g/L时，大分子木质素的去除率达89.6%，小分子木质素的去除率为26.1%，总糖损失率仅0.98%，表明复合蛋白质可优先去除大分子木质素，且对糖类物质影响很小。机制分析表明，蛋白质与木质素间主要通过氢键和疏水作用结合，当反应温度升高时，蛋白质分子间交联程度增大，形成尺寸更大、疏水性更强的聚集体，可进一步增强其对木质素的去除能力。 |
| Abstract: |
| In this study， a protein complex composed of egg white protein and soy protein isolate was used to adsorb the lignin in the hydrolysate obtained by the hydrothermal pretreatment of corn stover， and the effectiveness and mechanism of the protein complex in selective adsorption of lignin from the hydrolysate were investigated. The results showed that at reaction temperature of 95 ℃， pH value of 5， and a protein complex dosage of 1.5 g/L， the removal rate of macromolecular lignin reached 89.6%， the removal rate of small molecular lignin was 26.1%， and the total sugar loss was only 0.98%， indicating that the protein complex could preferentially remove macromolecular lignin while exerting minimal influence on sugars. Mechanism analysis revealed that the binding between the proteins and lignin was primarily driven by hydrogen bonding and hydrophobic interactions. As the reaction temperature increased， the degree of protein cross-linking increased， leading to the formation of larger aggregates with enhanced hydrophobicity， which further improved its lignin removal ability. |
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