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| Adsorption Behaviors of Cellulase on Cellulose Fiber Fractions with Different Fiber Length |
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| DOI：10.11981/j.issn.1000-6842.2011.02.37 |
| Key Words:cellulase; different fiber length; adsorption; adsorption isotherms; thermodynamics parameters |
| Fund Project:本项目得到国家973计划（2007CB210201）和国家建设高水平大学公派联合培养博士研究生项目的资助。 |
| Author Name | Affiliation | | 吕 健 | 华南理工大学制浆造纸工程国家重点实验室，广东广州，510640 | | 詹怀宇 | 华南理工大学制浆造纸工程国家重点实验室，广东广州，510640 | | 付时雨 | 华南理工大学制浆造纸工程国家重点实验室，广东广州，510640 |
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| Abstract: |
| Adsorption of cellulase on the surfaces of cellulose fibers is the first step of cellulose enzymatic hydrolysis and saccharification. The adsorption behaviors of cellulase on cellulose fiber fractions with different fiber length were quantitatively discussed in this study. The adsorption equilibrium was reached in around 60 minutes, and the adsorption amount increased with increasing initial cellulase dose. The adsorption process was well described by Langmuir isotherm. The maximum adsorption amount was found on the short fiber, and there was the maximum Langmuir adsorption equilibrium constant in the adsorption on the long fiber, which indicated that cellulase shows the highest adsorption affinity on the long fiber. The calculated thermodynamics parameters indicated that adsorption of cellulase on cellulose fiber is a spontaneous, exothermic and irreversible process. The highest enthalpy change ΔH° and entropy change ΔS° were found in the adsorption on 48 mesh fraction and 28 mesh fraction respectively. |
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