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| Chemical Structure and Antioxidant Activity of Different Sources of Lignin |
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| DOI：10.11981/j.issn.1000-6842.2019.04.14 |
| Key Words:lignin; structural analysis; antioxidant activity |
| Fund Project:青年科学基金项目（31800498）。 |
| Author Name | Affiliation | | ZHU Mengni | School of Light Industry and Chemical Engineering, Dalian Polytechnic University, Dalian, Liaoning Province, 116034 | | TIAN Weizhen | Anhui Jinnan Medical Technology Co., Ltd., Hefei, Anhui Province, 230601 | | WANG Xing | School of Light Industry and Chemical Engineering, Dalian Polytechnic University, Dalian, Liaoning Province, 116034 | | ZHOU Jinghui | School of Light Industry and Chemical Engineering, Dalian Polytechnic University, Dalian, Liaoning Province, 116034 | | LI Yao | School of Light Industry and Chemical Engineering, Dalian Polytechnic University, Dalian, Liaoning Province, 116034 |
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| Abstract: |
| In this paper, four kinds of agricultural residue, including corn stalk cellulose ethanol residue, reed, walnut shell and rice hull, were used as raw materials to extract lignin by NaOH method. The chemical structure of lignin was analyzed by gel permeation chromatography (GPC), Fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance spectroscopy (NMR). The results suggested that the total phenolic hydroxyl content of lignin sample was increased with H-type structural unit content increasing. Furthermore, there was positive correlation between the total phenolic hydroxyl content of lignin with its oxidation resistance including DPPH free radical scavenging ability and reducing capacity. When the concentration of four lignin samples was 0.4 mg/mL, the corresponding DPPH free radical scavenging abilities of the lignin samples were between 14.9% and 55.5%. Interestingly, all these lignin samples could enhance the growth of mouse osteoblasts. Especially, the mouse osteoblasts cell viability of the corn stalk cellulose ethanol residue lignin could reach to 710% at a concentration of 3.2 mg/mL. |
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