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| Determination of 5-Hydroxymethylfurfural in Pre-hydrolysate of Eucalyptus by UPLC-PDA Method |
| Received:May 31, 2019 |
| DOI：10.11981/j.issn.1000-6842.2020.03.40 |
| Key Words:5-hydroxymethylfurfural;UPLC;photo-diode array;pre-hydrolysis;Eucalyptus |
| Fund Project:国家自然科学基金（31370584）；2018年辽宁省高等学校创新人才项目。 |
| Author Name | Affiliation | Postcode | | QI Yungeng* | Liaoning Provincial University Key Laboratory of Pulp and Paper Engineering， School of Light Industry & Chemical Engineering， Dalian Polytechnic University， Dalian， Liaoning Province， 116034 | 116034 | | MA Mingshuai | Liaoning Provincial University Key Laboratory of Pulp and Paper Engineering， School of Light Industry & Chemical Engineering， Dalian Polytechnic University， Dalian， Liaoning Province， 116034 | 116034 | | GUO Huaize | Liaoning Provincial University Key Laboratory of Pulp and Paper Engineering， School of Light Industry & Chemical Engineering， Dalian Polytechnic University， Dalian， Liaoning Province， 116034 | 116034 | | LI Haiming | Liaoning Provincial University Key Laboratory of Pulp and Paper Engineering， School of Light Industry & Chemical Engineering， Dalian Polytechnic University， Dalian， Liaoning Province， 116034 | 116034 |
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| Abstract: |
| A method was developed to determine 5-hydroxymethylfurfural （5-HMF） in the pre-hydrolysate of Eucalyptus by Ultra Performance Liquid Chromatography-Photo-diode Array Detector （UPLC-PDA）. Without complicated pretreatment， different components in the samples were eluted and then separated with the mobile phase of acetonitrile/water （20/80，V/V） at flow rate of 0.2 mL/min， and then quantitatively detected with PDA detector at 283.55 nm. Detection limit， the correlation coefficient， relative standard deviation （RSD） and 5-HMF standard recovery rate of the determination method were 3.883 μg/L， 0.9998， 0.2% and 105.2%， respectively. The method developed had characteristics of simple sample preparation， fast determination and high accuracy. Finally， this method was used for fast determination of 5-HMF in the Eucalyptus pre-hydrolysate， which was beneficial to optimization of pre-hydrolysis conditions and utilization of pre-hydrolysate. |
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