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| Adsorption Effects of Lignin on Different Enzyme Proteins |
| Received:November 17, 2019 |
| DOI：10.11981/j.issn.1000-6842.2020.04.23 |
| Key Words:lignin;cellulase;xylanase;β-glycosidase;adsorption interaction |
| Fund Project:国家自然科学基金（31600469）；广东省自然科学基金（2017A030332343）；制浆造纸工程国家重点实验室2020年度开放基金（202010）。 |
| Author Name | Affiliation | E-mail | | MOU Hongyan* | State Key Lab of Pulp and Paper Engineering， South China University of Technology， Guangzhou， Guangdong Province， 510640
National Experimental Teaching Demonstration Center for Light Industry and Food， South China University of Technology， Guangzhou， Guangdong Province， 510640 | fehymou@scut.edu.cn | | LIU Yibei | State Key Lab of Pulp and Paper Engineering， South China University of Technology， Guangzhou， Guangdong Province， 510640
National Experimental Teaching Demonstration Center for Light Industry and Food， South China University of Technology， Guangzhou， Guangdong Province， 510640 | | | HUANG Jin | State Key Lab of Pulp and Paper Engineering， South China University of Technology， Guangzhou， Guangdong Province， 510640
National Experimental Teaching Demonstration Center for Light Industry and Food， South China University of Technology， Guangzhou， Guangdong Province， 510640 | |
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| Abstract: |
| Two kinds of lignin obtained from alkali and water soluble pretreatment of eucalyptus were used as raw materials， and through DNS and pNPG colorimetry， the effects of two kinds of lignin on the adsorption and desorption of different enzyme proteins （cellulase， xylanase， and β-glucosidase） and the inhibition of lignin adsorption on the activities of different enzyme proteins were studied. The results showed that the effect of temperature on the adsorption capacity of alkali lignin was greater than that of odium xylene sulfonate （SXS） lignin. The experiments of different enzyme proteins adsorbed on two kinds of lignin showed that the adsorbed enzyme proteins on SXS lignin were more easily to be desorbed. After lignin adsorption， the enzyme activity was significantly reduced， and the higher temperature could not only enhance the adsorption capacity of the lignins to enzyme proteins， but also inhibit the activity of enzyme. |
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