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Preparation of a Novel Cellulose-based Adsorbent and Its Immobilization Performance Towards Laccase
Received:October 15, 2021  
DOI:10.11981/j.issn.1000-6842.2022.03.01
Key Words:cellulose-based;graft modification;laccase immobilization;thermal stabilities;reusability
Fund Project:国家自然科学基金项目(31800502);广西清洁化制浆造纸与污染控制重点实验室开放基金项目(KF201804-5);“兴辽英才计划”科技创新领军人才项目(XLYC1802025)。
Author NameAffiliationPostcode
LYU Jingsi School of Light Industry & Chemical Engineering, Dalian Polytechnic University, Dalian, Liaoning Provine, 116034 116034
LU Peng Guangxi Key Laboratory of Clean Pulp and Paper and Pollution Control, College of Light Industry and Food Engineering, Guangxi University, Nanning, Guangxi Zhuang Autonomous Region, 530004 530004
XIAO Xingxiao School of Light Industry & Chemical Engineering, Dalian Polytechnic University, Dalian, Liaoning Provine, 116034 116034
PENG Chuanbo School of Light Industry & Chemical Engineering, Dalian Polytechnic University, Dalian, Liaoning Provine, 116034 116034
LI Na School of Light Industry & Chemical Engineering, Dalian Polytechnic University, Dalian, Liaoning Provine, 116034
Guangxi Key Laboratory of Clean Pulp and Paper and Pollution Control, College of Light Industry and Food Engineering, Guangxi University, Nanning, Guangxi Zhuang Autonomous Region, 530004 
530004
PING Qingwei School of Light Industry & Chemical Engineering, Dalian Polytechnic University, Dalian, Liaoning Provine, 116034 116034
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Abstract:
      A novel modified cellulose-based adsorbent was prepared in this study: cellulose was dissolved in NaOH/urea solution with addition of nano-CaCO3, which was then regenerated in HCl solution to prepare cellulose microsphere with porous structure. The as-prepared cellulose microsphere was further modified with acrylamide, leading to graft of carbon chain and introduction of acylamino which would be hydrolyzed to carboxyl under alkaline condition. The modified cellulose adsorbent immobilized laccase through electrostatic adsorption and hydrogen bond. The optimal conditions were duration of 6 h, temperature 50℃, monomer ratio 8∶5 for graft modification and pH value of 5.0, initial laccase concentration 10 g/L, time 3 h for immobilization of laccase. Under such conditions, laccase immobilized on the modified cellulose microspheres showed a significantly improved enzymatic activity by 52%. Moreover, good performances on thermal stability and reusability of immobilized laccase were obtained. The results indicate that the modified cellulose microsphere, as green-based carrier, possesses promising application prospects in laccase immobilization.
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