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Research Progress in Interactions Between Lignin and Cellulase and Its Inhibition on Cellulose Enzymatic Hydrolysis
Received:September 26, 2021  
DOI:10.11981/j.issn.1000-6842.2022.03.135
Key Words:lignin;cellulase;non-covalent interaction;cation-π interaction;lignin-blocking agent
Fund Project:国家自然科学基金(31870559和31901262);福建省自然科学基金(2018J01590和2019J01387);福建农林大学杰出青年科研人才计划项目(xjq201923);南京林业大学江苏省制浆造纸科学与技术重点实验室开放基金(KL201911)。
Author NameAffiliationPostcode
LI Dalin College of Materials Engineering, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, 350002 350002
HUANG Liulian College of Materials Engineering, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, 350002
National Forestry & Grassland Administration Key Laboratory for Plant Fiber Functional Materials, Fuzhou, Fujian Province, 350002 
350002
CHEN Lihui College of Materials Engineering, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, 350002
National Forestry & Grassland Administration Key Laboratory for Plant Fiber Functional Materials, Fuzhou, Fujian Province, 350002 
350002
LUO Xiaolin College of Materials Engineering, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, 350002
National Forestry & Grassland Administration Key Laboratory for Plant Fiber Functional Materials, Fuzhou, Fujian Province, 350002
Jiangsu Provincial Key Laboratory of Pulp and Paper Science and Technology, Nanjing Forestry University, Nanjing, Jiangsu Province, 210037 
210037
LIU Jing College of Materials Engineering, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, 350002
National Forestry & Grassland Administration Key Laboratory for Plant Fiber Functional Materials, Fuzhou, Fujian Province, 350002 
350002
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Abstract:
      Pretreatment is an effective way to deconstruct the dense structure of lignocellulose and improve the accessibility of cellulose to enzymes. After pretreatment, the residual lignin causes serious non-productive adsorption towards cellulase, hence it is essential to explore the interaction mechanism between lignin and cellulase so as to reduce the abovementioned adsorption. The electrostatic, hydrophobic, hydrogen bond, and cation-π interactions between lignin and cellulase and their effects on enzymatic hydrolysis efficiency of pretreated solid substrate were emphatically reviewed in this paper. Based on the lignin-cellulase interaction mechanism, some surfactants, metal ions, and non-catalytic proteins were applied as lignin-blocking agents, which could effectively overcome the adverse effects of lignin and significantly improve the enzymatic hydrolysis efficiency of pretreated solid substrate. However, the non-covalent interaction system between lignin and cellulase such as anion-π, π-π stacking, and p-π conjugative effect, need to be further explored. In general, systematically and comprehensively analyzing the lignin-cellulase mechanism can provide a theoretical basis for developing lignin-shielding agents and modifying the enzymes, and improving pretreatment efficiency, promoting the development of the lignocellulosic fuel chemical industry.
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