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| Study on Ternary DES Pretreated Poplar Sawdust for Boosting Enzymatic Hydrolysis of Cellulose |
| Received:August 29, 2022 |
| DOI：10.11981/j.issn.1000-6842.2022.04.25 |
| Key Words:deep eutectic solvent;pretreatment;enzymatic hydrolysis;crystallinity;crystallization index |
| Fund Project:江苏省高等学校大学生创新创业训练计划项目（202110298184H）；江苏省高等学校自然科学研究面上项目（20KJB220009）。 |
| Author Name | Affiliation | Postcode | | GUAN Chunlong* | College of Light Industry and Food Engineering， Nanjing Forestry University， Nanjing， Jiangsu Province， 210037 | 210037 | | XU Yexuan | College of Light Industry and Food Engineering， Nanjing Forestry University， Nanjing， Jiangsu Province， 210037 | 210037 | | BIAN Huiyang | College of Light Industry and Food Engineering， Nanjing Forestry University， Nanjing， Jiangsu Province， 210037 | 210037 | | ZHOU Xuelian | College of Light Industry and Food Engineering， Nanjing Forestry University， Nanjing， Jiangsu Province， 210037 | 210037 | | DAI Hongqi | College of Light Industry and Food Engineering， Nanjing Forestry University， Nanjing， Jiangsu Province， 210037 | 210037 |
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| Abstract: |
| A novel ternary deep eutectic solvent （DES） consisting of p-toluenesulfonic acid， choline chloride， and ethanol was used to pretreat poplar sawdust. The changes in chemical component， microscopic morphology， crystalline and chemical structure of poplar sawdust fiber during the ternary DES pretreatment process were investigated， as well as the effect of ternary DES pretreatment on the cellulose enzymatic hydrolysis. The results showed that the lignin removal rate of binary DES pretreated residues decreased rather than increased compared with that of raw poplar sawdust， while the lignin removal rate of ternary DES pretreated residues increased to 69.2%. The ternary DES system effectively inhibited the formation of acid-insoluble component on the surface of the residues， reduced invalid adsorption of cellulase， and significantly increased the efficiency of enzymatic hydrolysis to 76.6%. The destruction of hemicellulose， lignin， and amorphous cellulose during the ternary DES pretreatment resulted in the increase of crystallinity and crystallization index from 48.7% （poplar sawdust） to 65.7% and 0.66 （poplar sawdust） to 0.76， respectively. |
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