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| Purification and Characterization of Peroxidase from Fruit of Phenix roebelenii O. Brien for Catalytic Synthesis of Biobased-Dyes |
| Received:December 15, 2022 |
| DOI：10.11981/j.issn.1000-6842.2023.02.77 |
| Key Words:peroxidase;phenix roebelenii O. brien;catalysis;biobased-dyes |
| Fund Project:广东省自然科学基金（2017A030310341）；中央高校基本科研业务费（2022ZYGXZR0073）。 |
| Author Name | Affiliation | Postcode | | LIU Jingwen* | State Key Lab of Pulp and Paper Engineering， South China University of Technology， Guangzhou， Guangdong Province， 510640 | 510640 | | PENG Yangyang | Vegetable Research Institute， Guangdong Academy of Agricultural Sciences/ Guangdong Key Lab for New Technology Research of Vegetables， Guangzhou， Guangdong Province， 510640 | 510640 | | FU Shiyu | State Key Lab of Pulp and Paper Engineering， South China University of Technology， Guangzhou， Guangdong Province， 510640
South China University of Technology- Zhuhai Institute of Modern Industrial Innovation， Zhuhai， Guangdong Province， 519175 | 519175 | | LIU Ming | Vegetable Research Institute， Guangdong Academy of Agricultural Sciences/ Guangdong Key Lab for New Technology Research of Vegetables， Guangzhou， Guangdong Province， 510640 | 510640 | | LIU Hao | State Key Lab of Pulp and Paper Engineering， South China University of Technology， Guangzhou， Guangdong Province， 510640
South China University of Technology- Zhuhai Institute of Modern Industrial Innovation， Zhuhai， Guangdong Province， 519175 | 519175 |
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| Abstract: |
| Crude peroxidase was extracted from the fruit of Phenix roebelenii O. Brien， and purified by acetone precipitation， ion exchange chromatography and ultrafiltration. P. roebelenii Peroxidase （PRP） of electrophoretic purity was finally obtained with a molecular weight of 39670. The optimal catalytic conditions for guaiacol were pH value 5 and 60 ℃. By studying the effects of different metal ions， organic solvents and surfactants on PRP activity， it was found that Span-80 of 1 g/L could improve the activity of PRP at room temperature， pH value 5， Mn2+ or ethanol. Using guaiacol， 3，3'-dimethoxybenzidine and catechol as substrates， the Michaelis constant of PRP were determined to be 1.053， 5.563 and 2.316 mmol/L， respectively. Black， orange-red and brown-red dyes were obtained with PRP catalyzed catechol， catechin， guaiacol and 3，3'-dimethoxybenzidine， respectively. After being modulated with cellulose derivatives， it could be used for screen printing. The printing density of black water-based ink on A4 paper could reach 0.72. The peroxidase extracted from Phenix roebelenii O. Brien fruit is a green biocatalyst for synthesizing bio-based printing dyes. |
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