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Preparation of Cellulose Aerogel@ZIF-8 Composite by In-situ Growth Method and Its Immobilization for Lysozyme
Received:July 11, 2022  
DOI：10.11981/j.issn.1000-6842.2023.03.49
Key Words:cellulose;aerogel;in-situ;metal-organic frameworks;immobilization strategy
Fund Project:陕西省大学生创新创业训练计划（S202110708104）。
Author NameAffiliationPostcode
YANG Yuxuan* College of Bioresources Chemical and Materials Engineering， National Demonstration Center for Experimental Light Chemistry Engineering Education， Shaanxi Province Key Lab of Papermaking Technology and Specialty Paper， Key Lab of Auxiliary Chemistry & Technology for Chemical Industry， Ministry of Education， Shaanxi University of Science & Technology， Xi'an， Shaanxi Province， 710021 710021
WANG Yue College of Bioresources Chemical and Materials Engineering， National Demonstration Center for Experimental Light Chemistry Engineering Education， Shaanxi Province Key Lab of Papermaking Technology and Specialty Paper， Key Lab of Auxiliary Chemistry & Technology for Chemical Industry， Ministry of Education， Shaanxi University of Science & Technology， Xi'an， Shaanxi Province， 710021 710021
LIU Xueqian College of Bioresources Chemical and Materials Engineering， National Demonstration Center for Experimental Light Chemistry Engineering Education， Shaanxi Province Key Lab of Papermaking Technology and Specialty Paper， Key Lab of Auxiliary Chemistry & Technology for Chemical Industry， Ministry of Education， Shaanxi University of Science & Technology， Xi'an， Shaanxi Province， 710021 710021
CHENG Zhuqing College of Bioresources Chemical and Materials Engineering， National Demonstration Center for Experimental Light Chemistry Engineering Education， Shaanxi Province Key Lab of Papermaking Technology and Specialty Paper， Key Lab of Auxiliary Chemistry & Technology for Chemical Industry， Ministry of Education， Shaanxi University of Science & Technology， Xi'an， Shaanxi Province， 710021 710021
HE Xiaolong College of Bioresources Chemical and Materials Engineering， National Demonstration Center for Experimental Light Chemistry Engineering Education， Shaanxi Province Key Lab of Papermaking Technology and Specialty Paper， Key Lab of Auxiliary Chemistry & Technology for Chemical Industry， Ministry of Education， Shaanxi University of Science & Technology， Xi'an， Shaanxi Province， 710021 710021
QIAN Liwei College of Bioresources Chemical and Materials Engineering， National Demonstration Center for Experimental Light Chemistry Engineering Education， Shaanxi Province Key Lab of Papermaking Technology and Specialty Paper， Key Lab of Auxiliary Chemistry & Technology for Chemical Industry， Ministry of Education， Shaanxi University of Science & Technology， Xi'an， Shaanxi Province， 710021 710021
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Abstract:
      In recent years， with the rapid development of biochemistry and life sciences， the enzyme engineering has become a research hotspot. However， free enzymes have greatly limited its development and application due to its disadvantages such as sensitive structure， easy inactivation and failure， and difficulty in recycling. Immobilization of enzymes into specific carriers by means of adsorption or encapsulation can improve enzyme activity and stability， making further development possible. In this study， structurally regular cellulose aerogel@ZIF-8 composites were prepared using in situ growth method and lysozyme was successfully immobilized by multiple interactions between ZIF-8 and protein. Subsequently， the effects of pH value， NaCl concentration， adsorption time and adsorption concentration on the adsorption performance of the composites on the immobilization performance were investigated. The experimental results showed that the immobilization amount of cellulose aerogel@ZIF-8 lysozyme was 111.3 mg/g， the kinetics equilibrium can be completed within 40 minutes. In addition， the results of the catalytic experiments revealed that lysozyme immobilized by cellulose aerogel@ZIF-8 had better catalytic effect compared to the free enzyme. This study provides new ideas for the design and synthesis of sustainable and high performance immobilized materials.
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