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| Improving Furfural Tolerance of Saccharomyces cerevisiae by Adaptive Evolution and Its Mechanism |
| Received:August 06, 2022 |
| DOI：10.11981/j.issn.1000-6842.2023.04.24 |
| Key Words:Saccharomyces cerevisiae;furfural;adaptive evolution;ethanol |
| Fund Project:湖北省大学生创新创业项目（20200100020）；湖北省教育厅重点项目（21466000）。 |
| Author Name | Affiliation | Postcode | | LI Ziyin* | School of Bioengineering and Food， Hubei University of Technology， Wuhan， Hubei Province， 430068 | 430068 | | DU Wenshuang | School of Bioengineering and Food， Hubei University of Technology， Wuhan， Hubei Province， 430068 | 430068 | | LI Mengyan | School of Bioengineering and Food， Hubei University of Technology， Wuhan， Hubei Province， 430068 | 430068 | | PENG Jiajing | School of Bioengineering and Food， Hubei University of Technology， Wuhan， Hubei Province， 430068 | 430068 | | YAO Lan | School of Bioengineering and Food， Hubei University of Technology， Wuhan， Hubei Province， 430068 | 430068 |
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| Abstract: |
| Bioethanol could be produced from various raw materials and has little impact on environmental pollution. It is truly a renewable green energy. In order to increase the industrialization process of bioenergy and overcome the inhibitory effect of various inhibitors produced in the pretreatment process on yeast， here Saccharomyces cerevisiae CEN. PK 1B-7D was used as the starting strain for adaptive evolution. An evolutionary strain with highest tolerance to furfural was obtained and named as CEN. PK 1B-7D 385. Further analysis showed that the permeability of cell membrane of the evolved strain was decreased. The number of PI stained cells was decreased by 45.2%， and the number of depolarized cells was decreased by 66.7%， indicating that cell membrane of S. cerevisiae after adaptive evolution were more complete under furfural stress. At the same time， the intracellular ROS content of the evolved strain was decreased from 9.1% of the original strain to 6.5% of the evolved strain. Combined with biomass， ethanol yield， cell membrane permeability and membrane potential， the evolved strain CEN PK 1B-7D 385 is more tolerant to furfural than the starting strain. |
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