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| Isolation and Characterization of Lignin-based on Complete Dissolution System |
| Received:November 10, 2023 Revised:January 13, 2024 |
| DOI：10.11981/j.issn.1000-6842.2024.02.17 |
| Key Words:lignin;LiCl/DMSO;enzymatic hydrolysis lignin;poplar wood |
| Fund Project:国家自然科学基金（32271797，32271811）；南京林业大学大学生创新训练计划项目（2023NFUSPITP0597）。 |
| Author Name | Affiliation | Postcode | | LIU Yirun | College of Light Industry and Food Engineering， Nanjing Forestry University， Nanjing，Jiangsu Province， 210037 | 210037 | | WANG Xinhua | College of Light Industry and Food Engineering， Nanjing Forestry University， Nanjing，Jiangsu Province， 210037 | 210037 | | XU Huaiyu | College of Light Industry and Food Engineering， Nanjing Forestry University， Nanjing，Jiangsu Province， 210037 | 210037 | | JIN Yongcan | College of Light Industry and Food Engineering， Nanjing Forestry University， Nanjing，Jiangsu Province， 210037
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources， Nanjing Forestry University， Nanjing， Jiangsu Province， 210037 | 210037 | | WU Wenjuan* | College of Light Industry and Food Engineering， Nanjing Forestry University， Nanjing，Jiangsu Province， 210037
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources， Nanjing Forestry University， Nanjing， Jiangsu Province， 210037 | 210037 |
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| Abstract: |
| In order to obtain the complete chemical structure of lignin and comprehensively and profoundly analyze the structural characteristics of lignin， using poplar wood with ball milling for 2 h as raw material， the dissolving regenerative enzymolysis poplar lignin （DEL） was obtained by treatment of LiCl/DMSO system dissolution and regeneration， enzymatic hydrolysis， followed by extraction and purification. Compared with single cellulose enzymosis poplar lignin （CEL）， the yield and purity of DEL were 42.1% and 39 g/kg， respectively， which were higher than those of CEL. The results showed that the molecular weight of DEL was lower and its dispersity was better， and the lignin with lower molecular weight dissolved more during the separation process. The condensation degree of DEL was characterized by an alkaline nitrobenzene oxidation reaction， which further demonstrated that DEL was GS-type lignin， and the proportion of uncondensed structural units with condensation degree of lignin did not change during the separation process. |
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