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| Preparation and Application of Manganese-loaded Activated Carbon-Cellulose Composite Adsorbent for Enhancing Laccase Enzyme Activity |
| Received:August 08, 2023 Revised:October 25, 2023 |
| DOI：10.11981/j.issn.1000-6842.2024.03.38 |
| Key Words:waste manganese-loaded activated carbon;cellulose-based adsorbent;immobilized laccase;bisphenol A degradation |
| Fund Project:辽宁省教育厅面上项目（LJKMZ20220885）；生物基材料与绿色造纸国家重点实验室开放基金项目（GZKF202225）；天津市制浆造纸重点实验室开放基金项目（202106）。 |
| Author Name | Affiliation | Postcode | | LI Na | School of Light Industry and Chemical Engineering， Dalian Polytechnic University， Dalian， Liaoning Province， 116034 | 116034 | | MENG Xiangrui | School of Light Industry and Chemical Engineering， Dalian Polytechnic University， Dalian， Liaoning Province， 116034 | 116034 | | PENG Chuanbo | School of Light Industry and Chemical Engineering， Dalian Polytechnic University， Dalian， Liaoning Province， 116034 | 116034 | | YU Fangrui | School of Light Industry and Chemical Engineering， Dalian Polytechnic University， Dalian， Liaoning Province， 116034 | 116034 | | ZHANG Jian | School of Light Industry and Chemical Engineering， Dalian Polytechnic University， Dalian， Liaoning Province， 116034 | 116034 | | SHENG Xueru | School of Light Industry and Chemical Engineering， Dalian Polytechnic University， Dalian， Liaoning Province， 116034 | 116034 | | PING Qingwei | School of Light Industry and Chemical Engineering， Dalian Polytechnic University， Dalian， Liaoning Province， 116034 | 116034 |
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| Abstract: |
| A novel manganese-loaded activated carbon-cellulose composite adsorbent that could immobilize laccase and increase the enzyme activity was prepared by solvent regeneration method， using waste manganese-loaded activated carbon and cellulose， and further prepared the laccase-carrier material after immobilizing laccase. The results showed that the content of Mn2+ had a certain effect on the enzyme activity of the laccase-carrier material， and the enzyme activity of the laccase-carrier material was the highest when the content of Mn2+ in the composite adsorbent was 1.5 mg/g. The optimum process conditions for the immobilization of laccase were the immobilization temperature of 45 ℃， the pH value of 4， and the initial enzyme concentration of 6.5 g/L. The immobilization ratio of the laccase-carrier material was the highest to 48.24% under the optimum conditions of immobilization； the thermal and pH stabilities of the laccase-carrier material were significantly enhanced； the degradation rate of bisphenol A using the laccase-carrier material reached 68%， and the relative degradation rate of bisphenol A was still up to 51% after 5 times of reuse. |
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