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| Study on the Starch Enzymolysis Performance of α-Amylase and Its Complex Enzyme System |
| Received:April 03, 2024 Revised:April 17, 2024 |
| DOI：10.11981/j.issn.1000-6842.2024.03.87 |
| Key Words:modified starch;α-amylase;complex enzymes;enzymatic hydrolysis efficiency |
| Fund Project:国家十四五重点研发计划（2022YFC2105504）。 |
| Author Name | Affiliation | Postcode | | ZHU Hexin | Key Lab of Eco-Textiles， Ministry of Education， Jiangnan University， Wuxi， Jiangsu Province， 214122 | 214122 | | WANG Siwei | Key Lab of Eco-Textiles， Ministry of Education， Jiangnan University， Wuxi， Jiangsu Province， 214122 | 214122 | | HU Huijie | Key Lab of Eco-Textiles， Ministry of Education， Jiangnan University， Wuxi， Jiangsu Province， 214122 | 214122 | | ZHAO Ming | School of Bioengineering， Jiangnan University， Wuxi， Jiangsu Province， 214122 | 214122 | | LIU Nian | Wuxi School of Medicine， Jiangnan University， Wuxi， Jiangsu Province， 214122 | 214122 | | HU Jing | Wuxi School of Medicine， Jiangnan University， Wuxi， Jiangsu Province， 214122 | 214122 | | JIANG Yun | Key Lab of Eco-Textiles， Ministry of Education， Jiangnan University， Wuxi， Jiangsu Province， 214122 | 214122 | | DENG Haibo* | Key Lab of Eco-Textiles， Ministry of Education， Jiangnan University， Wuxi， Jiangsu Province， 214122 | 214122 |
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| Abstract: |
| This study investigated the enzymatic performance of single α-amylase and binary， ternary， and quaternary complex enzyme systems consisting of α-amylase with β-amylase， pullulanase and glucoamylase for three commonly used modified starches （oxidized starch， enzymatically hydrolyzed starch， and cationic starch）. The effect of different α-amylase dosages on starch enzymatic rate and DE value was explored. The results indicated that when the starch enzymatic rate reached 90% in single α-amylase system， the required amount of α-amylase for cationic starch enzymolysis was about 4 times than that of the other two kinds of starches. The enzymatic rate of enzymatically hydrolyzed starch could be increased by up to 37.4% when citric acid was added to inhibit zinc ions. In the binary complex enzyme system， the enzymatic rate of cationic starch by α-amylase/β-amylase was 5.3% and 8.7% higher than that by α-amylase/glucoamylase and α-amylase/pullulanase， respectively. In the ternary complex enzyme system， the enzymatic rate of cationic starch by α-amylase/glucoamylase/β-amylase was 1.2% higher than that by α-amylase/glucoamylase/pullulanase. In the application of α-amylase/glucoamylase/β-amylase/pullulanase tetrameric complex enzyme system to the enzymatic reactions of cationic starch， enzymatically hydrolyzed starch and oxidized starch， when the enzymatic rate of starch reached 80%， the required amount of α-amylase was 56%， 38% and 17% of that by single α-amylase system， respectively. |
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