基于MOFs复合木质海绵的蛋白印迹材料用于糖蛋白的高效和选择性分离

张煜昊; 杨苗秀; 刘振华; 杨雨璇; 郭淳; 杨梦浩; 黄举; 张景雯; 陈丽卿; 钱立伟

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Protein Imprinted Materials Based on MOFs Composite Wood Sponges for Efficient and Selective Separation of Glycoproteins

Received:July 21, 2023 Revised:July 26, 2023

DOI：10.11981/j.issn.1000-6842.2024.04.107

Key Words:wood sponge;metal-organic framework;glycoprotein;surface imprinting technique;protein imprinted polymer

Fund Project:国家自然科学基金（22323083）；陕西省外国专家服务计划重点项目（2023WGZJ-2D-11）。

Author Name	Affiliation	Postcode
ZHANG Yuhao	College of Bioresources Chemical & Materials Engineering， Shaanxi University of Science & Technology， Xi’an， Shaanxi Province， 710021	710021
YANG Miaoxiu^*	China National Pulp and Paper Research Institute Co.， Ltd.， Beijing， 100102	100102
LIU Zhenhua	China National Pulp and Paper Research Institute Co.， Ltd.， Beijing， 100102	100102
YANG Yuxuan	College of Bioresources Chemical & Materials Engineering， Shaanxi University of Science & Technology， Xi’an， Shaanxi Province， 710021	710021
GUO Chun	School of Culture and Education， Shaanxi University of Science and Technology， Xi’an， Shaanxi Province， 710021	710021
YANG Menghao	College of Bioresources Chemical & Materials Engineering， Shaanxi University of Science & Technology， Xi’an， Shaanxi Province， 710021	710021
HUANG Ju	China National Pulp and Paper Research Institute Co.， Ltd.， Beijing， 100102	100102
ZHANG Jingwei	China National Pulp and Paper Research Institute Co.， Ltd.， Beijing， 100102	100102
CHEN Liqing	China National Pulp and Paper Research Institute Co.， Ltd.， Beijing， 100102	100102
QIAN Liwei^*	College of Bioresources Chemical & Materials Engineering， Shaanxi University of Science & Technology， Xi’an， Shaanxi Province， 710021	710021

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Abstract:

The efficient enrichment and identification of glycoproteins hold significant relevance to proteomics-related medical and biological research. However， constructing high-performance glycoprotein imprinted materials remains challenging. In this study， the wood sponge@ UIO-66 composite （WCS@UIO-66） was employed as a carrier， and 4-vinylphenylboronic acid was taken as a covalent functional monomer to design and synthesize horseradish peroxidase-imprinted materials （WCS@UIO-66@MIPs）. Utilizing the porous structure and abundant functional groups of the wood sponge and metal-organic framework， the immobilization of template proteins was facilitated while reducing the mass transfer resistance. Consequently， WCS@UIO-66@MIPs not only demonstrated exceptionally high adsorption capacity （182.0 mg/g） and excellent selectivity （Imprinting Factor=2.68） towards horseradish peroxidase， but also achieved adsorption equilibrium within 60 min. Moreover， it could specifically capture target proteins in mixed protein solutions， demonstrating its promising potential applications for glycoprotein enrichment and identification.

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