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Effects of Ultra-low Concentration Acid Pretreatment on the Separation of Wheat Straw Components and Cellulose Enzymolysis Efficiency
Received:November 13, 2024  Revised:December 17, 2024
DOI:10.11981/j.issn.1000-6842.2025.02.01
Key Words:wheat straw;formic acid;pretreatment;enzyme hydrolysis
Fund Project:国家重点研发课题(2021YFC2101601);国家自然科学基金国际合作项目(22361132543)。
Author NameAffiliationPostcode
LIU Xueqin* School of Light Industry and Engineering, State Key Lab of Advanced Papermaking and Paper-based Materials, South China University of Technology, Guangzhou, Guangdong Province, 510640 510640
LYU Yingren School of Light Industry and Engineering, State Key Lab of Advanced Papermaking and Paper-based Materials, South China University of Technology, Guangzhou, Guangdong Province, 510640 510640
ZHU Ruonan School of Light Industry and Engineering, State Key Lab of Advanced Papermaking and Paper-based Materials, South China University of Technology, Guangzhou, Guangdong Province, 510640 510640
LIU Xiaohong School of Light Industry and Engineering, State Key Lab of Advanced Papermaking and Paper-based Materials, South China University of Technology, Guangzhou, Guangdong Province, 510640 510640
WANG Xingjie School of Light Industry and Engineering, State Key Lab of Advanced Papermaking and Paper-based Materials, South China University of Technology, Guangzhou, Guangdong Province, 510640 510640
REN Junli* School of Light Industry and Engineering, State Key Lab of Advanced Papermaking and Paper-based Materials, South China University of Technology, Guangzhou, Guangdong Province, 510640 510640
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Abstract:
      In this study, the effects of ultra-low concentration acid types and pretreatment conditions on the fractionation of wheat straw and enzymolysis were investigated. The results showed that formic acid pretreatment was the most effective. Under the pretreatment conditions of formic acid mass concentration of 1 g/L, the solid-liquid ratio of 1∶20, pretreatment temperature of 170 ℃, and time of 30 min, the 95.55% cellulose retention rate was achieved. The removal rate of hemicellulose and lignin were 81.66% and 38.42%, respectively, the total yield of xylose and xylo-oligosaccharide in the hydrolysate was 80.05%. In addition, the pretreatment residue obtained under this condition showed a glucose conversion of 83.37% after 72 h of enzymatic hydrolysis reaction. After two-step hydrolysis, the glucose and xylose yields was obtained to 95.32%.
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