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| In-situ Construction of a Wood Sponge with Laccase-like Activity and Cu-Nₓ Active Center for Adrenaline Colorimetric Detection |
| Received:May 19, 2025 Revised:June 05, 2025 |
| DOI：10.11981/j.issn.1000-6842.2025.03.66 |
| Key Words:wood sponge;amination;coordination;enzyme mimic;biosensing |
| Fund Project:制浆造纸国家工程实验室基金项目（ZZY2024QNRC02）；国家自然科学基金面上项目（22278035）；国家特殊海外高层次人才计划项目。 |
| Author Name | Affiliation | Postcode | | LI Lijun* | China National Pulp and Paper Research Institute Co.， Ltd.， Beijing， 100102
Beijing Key Lab of Lignocellulosic Chemistry， Beijing Forestry University， Beijing， 100083 | 100083 | | DUAN Tong | Beijing Key Lab of Lignocellulosic Chemistry， Beijing Forestry University， Beijing， 100083 | 100083 | | DU Fan | China National Pulp and Paper Research Institute Co.， Ltd.， Beijing， 100102 | 100102 | | CHEN Xuemei | China National Pulp and Paper Research Institute Co.， Ltd.， Beijing， 100102 | 100102 | | YANG Guangzhao | China National Pulp and Paper Research Institute Co.， Ltd.， Beijing， 100102 | 100102 | | ZHANG Xue* | China National Pulp and Paper Research Institute Co.， Ltd.， Beijing， 100102 | 100102 | | ZHANG Xueming* | Beijing Key Lab of Lignocellulosic Chemistry， Beijing Forestry University， Beijing， 100083 | 100083 |
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| Abstract: |
| The catalytic oxidation of epinephrine to quinones by laccase or its mimics is pivotal for the colorimetric detection of epinephrine. To address the inherent limitations of natural laccase， such as high cost， instability， and the tendency of nanozymes to aggregate during this process， the natural balsa wood was modified through amination， carbonization， and coordination with Cu²⁺ in this study. This approach enabled the in-situ construction of a wood sponge （Cu-WS） featuring laccase-like Cu-Nₓ active sites. The stable coordination structure formed between the aminated cellulose， hemicellulose， lignin， and Cu²⁺ endowed Cu-WS with catalytic properties closely resembling natural laccase. Specifically， its Michaelis constant （Kₘ） for 2，4-dichlorophenol （2，4-DCP） was 0.27 mmol/L， comparable to that of natural laccase （Km = 0.24 mmol/L）， and its maximum reaction velocity （vmax） was enhanced by 23 times. Remarkably， Cu-WS retained >50% catalytic relative activity after 210 d storage and >90% over 7 reuse cycles， outperforming natural laccase. For epinephrine detection， Cu-WS achieved high affinity （Km = 4.07 × 10⁻⁶ mol/L） and catalytic efficiency （vmax = 1.04 × 10⁻6 mol/（L·s））， with a linear range of 5~30 μg/mL and LOD of 0.019 6 μg/mL. Leveraging the 3D porous wood framework for enhanced mass transfer and Cu-Nx-mediated biomimetic catalysis， Cu-WS enabled rapid， instrument-free colorimetric detection. |
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