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| Preparation and Application of Fractionated Small Molecular Lignin Potassium Fertilizer via KOH Gradient Dissolution |
| Received:September 12, 2025 Revised:January 14, 2026 |
| DOI：10.11981/j.issn.1000-6842.2026.02.01 |
| Key Words:black liquor;lignin;fractionation;potassium fertilizer;salt stress |
| Fund Project:天津市自然科学基金重点项目（24JCZDJC00780）；非粮生物质能技术全国重点实验室开放基金（SKL-NFBET-2025-22）；“协同”行动计划（广西区域创新能力提升计划）（桂科XT2600870040）。 |
| Author Name | Affiliation | Postcode | | YANG Ranran* | 1College of Light Industry Science and Engineering， Tianjin University of Science and Technology， Tianjin， 300457 | 300457 | | LI Wenhui | 1College of Light Industry Science and Engineering， Tianjin University of Science and Technology， Tianjin， 300457 | 300457 | | LI Baotao | 1College of Light Industry Science and Engineering， Tianjin University of Science and Technology， Tianjin， 300457 | 300457 | | LIU Yile | 1College of Light Industry Science and Engineering， Tianjin University of Science and Technology， Tianjin， 300457 | 300457 | | SUI Wenjie | 2State Key Lab of Food Nutrition and Safety， Tianjin University of Science and Technology， Tianjin， 300457 | 300457 | | LI Dongmin | 3COFCO Nutrition & Health Research Institute Co.， Ltd.， Beijing， 102209 | 102209 | | WANG Ning | 4Guangxi Lanke Resource Recycling and Utilization Co.， Ltd.， Yulin， Guangxi Zhuang Autonomous Region， 537000 | 537000 | | SI Chuanling | 1College of Light Industry Science and Engineering， Tianjin University of Science and Technology， Tianjin， 300457 | 300457 | | WANG Guanhua* | 1College of Light Industry Science and Engineering， Tianjin University of Science and Technology， Tianjin， 300457 | 300457 |
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| Abstract: |
| Lignin was extracted from black liquor through acid precipitation， and a KOH gradient dissolution was employed to simultaneously achieve lignin fractionation and potassiumization， resulting in the synthesis of small molecular lignin potassium fertilizer (LMWL-K) with a high content of hydrophilic groups， which was subsequently applied in the cultivation of potassium-demanding butterhead lettuce seedlings. The results demonstrated that the small molecular lignin (LMWL) exhibited high contents of phenolic hydroxyl (2.98 mmol/g) and carboxyl groups (2.30 mmol/g). Consequently， LMWL-K achieved a potassium content of 15.9%， significantly higher than that of the full-spectrum lignin potassium fertilizer (10.3%). Hydroponic experiments on butterhead lettuce seedlings demonstrated that the optimal treatment efficacy was observed at an LMWL-K dosage of 150 mg/L. Compared with the control group without addition of potassium fertilizer application， LMWL-K significantly enhanced biomass accumulation， increasing the contents of chlorophyll a， chlorophyll b， soluble protein， and soluble sugar by 22.3%， 48.9%， 231%， and 40.5%， respectively. In addition to LMWL-K serving as a potassium source， the LMWL component in LMWL-K was found to stimulate root tip cell division， promote root development， and enhance nutrient uptake， thereby increasing seedling biomass. Its growth-promoting effects surpassed those of potassium humate and inorganic potassium fertilizers at equivalent potassium concentrations. Furthermore， LMWL-K was shown to regulate osmotic metabolism and activate the antioxidant enzyme system， thereby significantly enhancing the salt tolerance of the butterhead lettuce seedlings. |
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