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Study on the In-situ Bacterial Cellulose Synthesis Modification of Sludge Based on Distiller’s Grains Enzymatic Hydrolysate and Its Application as a Paper Filler
Received:January 21, 2026  Revised:February 06, 2026
DOI:10.11981/j.issn.1000-6842.2026.02.125
Key Words:bacterial cellulose;in-situ synthesis;distillers’ grains enzymatic hydrolysate;river silt modification;papermaking filler
Fund Project:无锡市建设系统科技项目(wx202502zd)。
Author NameAffiliationPostcode
WU Zhiyi* 1School of Mechanical Engineering, Jiangsu Key Lab of Advanced Food Manufacturing Equipment & Technology, Jiangnan University, Wuxi, Jiangsu Province, 214122 214122
WEI Lingjun 1School of Mechanical Engineering, Jiangsu Key Lab of Advanced Food Manufacturing Equipment & Technology, Jiangnan University, Wuxi, Jiangsu Province, 214122 214122
ZHOU Jiawei 1School of Mechanical Engineering, Jiangsu Key Lab of Advanced Food Manufacturing Equipment & Technology, Jiangnan University, Wuxi, Jiangsu Province, 214122 214122
YAN Xinchi 1School of Mechanical Engineering, Jiangsu Key Lab of Advanced Food Manufacturing Equipment & Technology, Jiangnan University, Wuxi, Jiangsu Province, 214122 214122
SUN Hao* 1School of Mechanical Engineering, Jiangsu Key Lab of Advanced Food Manufacturing Equipment & Technology, Jiangnan University, Wuxi, Jiangsu Province, 214122
2Tsinghua Suzhou Environmental Innovation Research Institute, Suzhou, Jiangsu Province, 215000 
215000
WANG Changkun 1School of Mechanical Engineering, Jiangsu Key Lab of Advanced Food Manufacturing Equipment & Technology, Jiangnan University, Wuxi, Jiangsu Province, 214122 214122
DU Tiantian 1School of Mechanical Engineering, Jiangsu Key Lab of Advanced Food Manufacturing Equipment & Technology, Jiangnan University, Wuxi, Jiangsu Province, 214122 214122
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Abstract:
      In this study, enzymatic hydrolysate of distiller’s grains was used to drive kombucha bacteria to in-situ construct bacterial cellulose (BC) networks on the surface of river silt, to prepare a green and low-cost papermaking fillers, and investigating its application effect in paper. The results showed that the composite filler (EW-BC/SP) prepared with the distiller’s grains enzymatic hydrolysate medium achieved a BC loading of 109.1 mg/g, exhibiting an excellent BC loading capacity with superior in-situ modification performance compared with the traditional medium group (68.6 mg/g). When the filler dosage was fixed at 40%, the retention rate of EW-BC/SP in paper reached as high as 88.2%, which was significantly higher than that of SP group (54.7%). The tensile index of the paper added EW-BC/SP reached 31.2 N·m/g, which was 30.6% higher than that of SP group, and the burst index was 1.81 kPa·m²/g, showing basically no difference from SP group, and demonstrated the remarkable enhancement effect of the BC network.
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