| 王银存,王卫卫,李利军,马英辉,王小娟,卢美欢.响应面法优化葡糖醋杆菌产细菌纤维素的发酵工艺[J].中国造纸学报,2012,27(2):45-49 |
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| 响应面法优化葡糖醋杆菌产细菌纤维素的发酵工艺 |
| Optimization of Gluconacetobacter Fermentation for Bacterial Cellulose Production by Response Surface Method |
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| DOI： |
| 中文关键词: 响应面法 细菌纤维素 发酵工艺 葡糖醋杆菌 |
| Key Words:response surface method bacterial cellulose fermentation Gluconacetobacter |
| 基金项目:陕西省科学院青年人才基金(2010k-29)。 |
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| 中文摘要: |
| 为提高葡糖醋杆菌生产细菌纤维素的产量,采用Plackett-Burman实验设计和Box-Benhnken Design相结合，对红薯酶解液、起始pH值、装液量等因素进行了研究，优化了产细菌纤维素的发酵工艺。实验结果表明，产细菌纤维素的最佳工艺为：红薯酶解液质量浓度50 g/L、起始pH值6.0、装液量50 mL/（250 mL）。该工艺条件下得到的细菌纤维素绝干质量（折算成质量浓度）达到4.80 g/L，比优化前产量2.20 g/L提高了118%。 |
| Abstract: |
| In order to increase bacterial cellulose production yielded by Gluconacetobacter, the factors including sweet potato hydrolyzate, initial pH, liquid volume in flask and so on by using Plackett-Burman Design and Box-Benhnken Design combination were studied, and the fermentation of bacterial cellulose production was optimized. The results showed that the best conditions for bacterial cellulose production were as follows: sweet potato hydrolyzate of 50 g/L, initial pH 6.0, liquid volume in flask of 50 mL/(250 mL). Under these conditions, the dry weight of the bacterial cellulose reached to 4.80 g/L and increased by 118% compared with 2.20 g/L before optimization. |
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