| 夏 青,姚 兰,张宏伟,杨海涛.硫酸盐浆酶解液中LCC的提取及性质研究[J].中国造纸学报,2015,30(3):5-9 |
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| 硫酸盐浆酶解液中LCC的提取及性质研究 |
| Isolation and Characteristic Research of LCC from Enzymatic Hydrolysis Liquor of Kraft Wheat Straw Pulp |
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| DOI：10.11981/j.issn.1000-6842.2015.03.5 |
| 中文关键词: 麦草浆 聚木糖酶 纤维素酶 2D-HSQC |
| Key Words:wheat straw pulp xylanase cellulase 2D-HSQC |
| 基金项目:国家自然科学基金（31500496）；绿色轻工材料湖北省重点实验室2013年开放基金。 |
| 作者 | 单位 | | 夏 青1 | 1.湖北工业大学轻工学部制浆造纸工程学院,湖北武汉,430068 | | 姚 兰1,2 | 1.湖北工业大学轻工学部制浆造纸工程学院,湖北武汉,430068；2.绿色轻工材料湖北省重点实验室,湖北武汉,430068 | | 张宏伟3 | 3.武汉联合工程顾问咨询有限公司,湖北武汉,430071 | | 杨海涛1,2,* | 1.湖北工业大学轻工学部制浆造纸工程学院,湖北武汉,430068；2.绿色轻工材料湖北省重点实验室,湖北武汉,430068 |
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| 中文摘要: |
| 为了探究硫酸盐浆在酶解过程中是否有小分子LCC溶出及其性质,以麦草为原料，通过硫酸盐法制浆得到硫酸盐麦草浆,并依次进行聚木糖酶、纤维素酶酶解。利用凝胶渗透色谱柱分离出酶解液中的小分子LCC,然后对LCC进行FT-IR、2D-HSQC(二维异核单量子碳氢相关)核磁共振分析。结果表明,从麦草浆聚木糖酶酶解液(XHL)中可分离出小分子LCC,但在纤维素酶酶解液(CHL)中只存在纤维素；2D-HSQC分析表明,从XHL中分离得到的小分子LCC主要是聚木糖和木素通过苯基配糖体键、苯甲醚键和γ-酯键连接在一起的,说明聚木糖酶能同时对纸浆中残余的聚木糖和木素与聚木糖之间的化学连接键进行降解。 |
| Abstract: |
| In the study, wheat straw kraft pulp was prepared, then it was treated by xylanase and cellulase sequentially. The low molecular weight LCC was isolated from the enzymatic hydrolysis liquor using Gel Penetration Chromatography column. FT-IR and 2D-HSQC were employed to study the characteristic of the LCC. The results showed that LCC existed only in xylanase hydrolysis liquor (XHL).The analysis results of 2D-HSQC indicated that the LCC was formed by xylan and lignin connected by benzyl ether, phenyl glycoside and γ-ester bonds.It could also conclude that in xylanase hydrolysis process, xylan and the linkages of lignin-xylan in the kraft pulp could be degraded. |
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