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| Optimization of Gluconacetobacter Fermentation for Bacterial Cellulose Production by Response Surface Method |
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| DOI： |
| Key Words:response surface method; bacterial cellulose; fermentation;Gluconacetobacter |
| Fund Project:陕西省科学院青年人才基金(2010k-29)。 |
| Author Name | Affiliation | | 王银存1,2 | 1.陕西省微生物研究所，陕西西安 710043
2.西北大学生命科学学院，陕西西安，710069 | | 王卫卫2 | 2.西北大学生命科学学院，陕西西安，710069 | | 李利军1 | 1.陕西省微生物研究所，陕西西安 710043 | | 马英辉1 | 1.陕西省微生物研究所，陕西西安 710043 | | 王小娟1,2 | 1.陕西省微生物研究所，陕西西安 710043
2.西北大学生命科学学院，陕西西安，710069 | | 卢美欢1,* | 1.陕西省微生物研究所，陕西西安 710043 |
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| Abstract: |
| In order to increase bacterial cellulose production yielded by Gluconacetobacter, the factors including sweet potato hydrolyzate, initial pH, liquid volume in flask and so on by using Plackett-Burman Design and Box-Benhnken Design combination were studied, and the fermentation of bacterial cellulose production was optimized. The results showed that the best conditions for bacterial cellulose production were as follows: sweet potato hydrolyzate of 50 g/L, initial pH 6.0, liquid volume in flask of 50 mL/(250 mL). Under these conditions, the dry weight of the bacterial cellulose reached to 4.80 g/L and increased by 118% compared with 2.20 g/L before optimization. |
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