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| Application of Statistical Design for the Construction of Multiple Microorganisms to Enhance Biodegradation of Pulp and Paper Effluent |
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| DOI：10.11981/j.issn.1000-6842.2013.03.42 |
| Key Words:multiple microorganisms; Plackett-Burman design; steepest ascent design; central composite design; response surface method |
| Fund Project:国家自然科学基金资助项目(20977033)；教育部留学回国人员科研启动基金资助项目；华南理工大学学生研究计划。 |
| Author Name | Affiliation | | 孙友友1 | 1.华南理工大学制浆造纸工程国家重点实验室，广东广州，510640 | | 陈元彩1,2,* | 1.华南理工大学制浆造纸工程国家重点实验室，广东广州，510640；2.华南理工大学环境科学与工程学院，广东广州，510006 | | 胡勇有2 | 2.华南理工大学环境科学与工程学院，广东广州，510006 |
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| Abstract: |
| The microorganisms with 9 bacterial strains were constructed and optimized to enhance the treatment efficiency of pulp and paper effluent by orderly using Plackett-Burman(PB) design, steepest ascent design, central composite design and response surface method. Firstly, Plackett-Burman design was used to find the strains having significant effect on the degradation of the pulp and paper effluent, i.e. Bacillus sp., PE-9, Pseudomonas putida, Xz6-1. Secondly, the maximum response region for the central composite design was determined using the method of steepest ascent. Finally, the optimal strains concentration (OD600) was determined using central composite design and response surface method analysis. The optimized multiple microorganisms contained Bacillus sp. (0.35), Enterobacter Cloacae（0.2），Gordonia sp.（0.3），Pseudomonas putida （0.38），PB-3（0.3），PE-9（0.41），PG-1（0.2），Ppp-3（0.3），Xz6-1（0.35）. Under such conditions, the CODCr degradation with multiple microorganisms was 86.9%, which was good agreement with the predicted one of (85.7±0.5)%. In conclusion, the construction of multiple microorganisms by a statistical experimental design has good reliability． |
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