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Isolation and Characteristic Research of LCC from Enzymatic Hydrolysis Liquor of Kraft Wheat Straw Pulp |
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DOI:10.11981/j.issn.1000-6842.2015.03.5 |
Key Words:wheat straw pulp; xylanase; cellulase; 2D-HSQC |
Fund Project:国家自然科学基金(31500496);绿色轻工材料湖北省重点实验室2013年开放基金。 |
Author Name | Affiliation | 夏 青1 | 1.湖北工业大学轻工学部制浆造纸工程学院,湖北武汉,430068 | 姚 兰1,2 | 1.湖北工业大学轻工学部制浆造纸工程学院,湖北武汉,430068;2.绿色轻工材料湖北省重点实验室,湖北武汉,430068 | 张宏伟3 | 3.武汉联合工程顾问咨询有限公司,湖北武汉,430071 | 杨海涛1,2,* | 1.湖北工业大学轻工学部制浆造纸工程学院,湖北武汉,430068;2.绿色轻工材料湖北省重点实验室,湖北武汉,430068 |
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Abstract: |
In the study, wheat straw kraft pulp was prepared, then it was treated by xylanase and cellulase sequentially. The low molecular weight LCC was isolated from the enzymatic hydrolysis liquor using Gel Penetration Chromatography column. FT-IR and 2D-HSQC were employed to study the characteristic of the LCC. The results showed that LCC existed only in xylanase hydrolysis liquor (XHL).The analysis results of 2D-HSQC indicated that the LCC was formed by xylan and lignin connected by benzyl ether, phenyl glycoside and γ-ester bonds.It could also conclude that in xylanase hydrolysis process, xylan and the linkages of lignin-xylan in the kraft pulp could be degraded. |
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