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| Study on Biomimetic Degradation of Residual Lignin in Kraft Pulp by 13C-2H Dual Isotope Tracer |
| Received:August 01, 2022 |
| DOI：10.11981/j.issn.1000-6842.2023.04.10 |
| Key Words:kraft pulping;residual lignin;dual isotope tracer;biomimetic degradation;ginkgo |
| Fund Project:国家自然科学基金项目（21878070）；湖北省高等学校优秀中青年科技创新团队计划项目（T201205）。 |
| Author Name | Affiliation | Postcode | | ZHANG Gongxia* | Research Institute of Pulp & Paper Engineering， Hubei University of Technology， Wuhan， Hubei Province， 430068 | 430068 | | LU Desheng | Research Institute of Pulp & Paper Engineering， Hubei University of Technology， Wuhan， Hubei Province， 430068 | 430068 | | CHEN Xudong | Research Institute of Pulp & Paper Engineering， Hubei University of Technology， Wuhan， Hubei Province， 430068 | 430068 | | YUE Yuanyuan | Research Institute of Pulp & Paper Engineering， Hubei University of Technology， Wuhan， Hubei Province， 430068 | 430068 | | XIE Yimin | Research Institute of Pulp & Paper Engineering， Hubei University of Technology， Wuhan， Hubei Province， 430068 | 430068 |
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| Abstract: |
| In this study， coniferin-［α-13C］ was synthesized， and then injected into a growing shoot of Ginkgo biloba L. together with the D-glucose-［6-2H2］ of polysaccharide precursor and inhibitor （carboxymethoxyamine hemihydrochloride， AOA） of phenylalanine ammonialyas， to obtain 13C and 2H double isotope-labeled ginkgo wood mill. The wood mill was cooked with kraft method and the obtained pulp was treated by enzymatic hydrolysis to obtain 13C and 2H double isotope labeled cellulolytic enzyme lignin（CEL）. The purified CEL was subjected to biomimetic treatment with GIF-sur-Yvette （GIF） and Cu（Salen） system which was composed of salicylaldehyde， ethylenediamine and Cu2+ to further remove lignin and lignin-carbohydrate complexes （LCC）. The residues after biomimetic treatment were analyzed by nuclear magnetic resonance （13C NMR and 1H NMR）. The results showed that the lignin structural units in the residues after GIF system biomimetic treatment were mainly connected with β-5， β-O-4 and β-1 substructures， while a small amount of lignin was connected with cellulose by acetal bond and benzyl ether linkage （about 6.7%）. The residual lignin structural units after biomimetic treatment by Cu（Salen） system were mainly connected with β-O-4 and β-1 structures. It was found that there was a small amount of cellulose after the biomimetic treatment in CEL， but no signal of the benzyl ether bond and acetal bond between lignin and cellulose was found. The 1H NMR differential spectrum only showed the signals of some unsubstituted polysaccharides， but the connection between lignin and polysaccharides was not found. The results showed that Cu（Salen） system biomimetic catalytic oxidation had relatively strong degradation ability for LCC. |
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