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| Study on Removing Lignin from Wheat Straw Alkali Pretreatment Hydrolysate by Thermal Flocculation of Egg White Proteins |
| Received:December 15, 2023 Revised:January 14, 2024 |
| DOI：10.11981/j.issn.1000-6842.2024.04.152 |
| Key Words:egg white proteins;flocculation;wheat straw alkali pretreatment hydrolysate;biodegradability |
| Author Name | Affiliation | Postcode | | CHEN Yan | Tianjin Key Lab of Pulp and Paper Industry， Tianjin University of Science and Technology， Tianjin， 300457 | 300457 | | LYU Pengjing | Tianjin Key Lab of Pulp and Paper Industry， Tianjin University of Science and Technology， Tianjin， 300457 | 300457 | | CHEN Yanbing | Tianjin Key Lab of Pulp and Paper Industry， Tianjin University of Science and Technology， Tianjin， 300457 | 300457 | | DING Wenrui | Tianjin Key Lab of Pulp and Paper Industry， Tianjin University of Science and Technology， Tianjin， 300457 | 300457 | | LI Min | Zhuhai Hongta Renheng Packaging Co.， Ltd.， Zhuhai， Guangdong Province， 519070 | 519070 | | LIU Chunlan | Shandong Kemai Biological Pulp Co.， Ltd.， Weifang， Shandong Province， 262400 | 262400 | | WANG Gaosheng | Tianjin Key Lab of Pulp and Paper Industry， Tianjin University of Science and Technology， Tianjin， 300457 | 300457 | | YU Menghui* | Tianjin Key Lab of Pulp and Paper Industry， Tianjin University of Science and Technology， Tianjin， 300457 | 300457 |
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| Abstract: |
| In order to improve the biodegradability of the alkali pretreatment hydrolysate， the lignin of the alkali pretreatment hydrolysate was removed by egg white proteins （EWPs） thermal flocculation process in this study. The method was compared with the EWPs flocculation processes at room temperature， and the traditional flocculation processes using FeCl3 and polyaluminum chloride （PAC） as flocculants， respectively. The results showed that when the concentration of the alkali pretreatment hydrolysate （calculated by CODCr） was 8 g/L， the removal rate of macromolecular colloidal lignin （calculated by turbidity） in the alkali pretreatment hydrolysate was 70.6%±2.2%， and the removal rate of small molecule soluble lignin （calculated by total phenol content） reached 20.7%±1.4% after EWPs thermal flocculation treatment. Compared with EWPs flocculation process at room temperature， FeCl3 and PAC flocculation processes， the removal rate of lignin increased. Investigation about the mechanism of lignin removal by EWPs thermal flocculation showed that high-temperature-induced denaturation of EWPs led to protein structural changes， promoting the bindings between EWPs and lignin resulted from the hydrophobic flocculation and hydrogen bonding. During the heating process， macromolecular flocs were further formed through hydrogen interaction and sulfhydryl-disulfide conversion reaction of EWPs protein molecular with lignin， which promoted the flocculation and removal of macromolecular lignin and its derivatives in the alkali pretreatment hydrolysate. |
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